| Leukocyte expression profiles reveal gene sets with prognostic value for seizure-free outcome following stereotactic laser amygdalohippocampotomy.
Sprissler R1,2, Bina R3, Kasoff W3, Witte MH4, Bernas M4,5, Walter C3, Labiner DM6, Lau B2, Hammer MF7,8, Weinand ME3.
uthor information
1Center for Applied Genetics and Genomic Medicine, University of Arizona, Tucson, AZ, 85721, USA.2Arizona Research Labs Division of Biotechnology, University of Arizona, Tucson, AZ, 85721, USA.3Division of Neurosurgery, Department of Surgery, University of Arizona College of Medicine, Tucson, AZ, 85724, USA.4Department of Surgery, University of Arizona College of Medicine, Tucson, AZ, 85724, USA.5University of North Texas Health Science Center, Fort Worth, TX, 76107, USA.6Department of Neurology, University of Arizona College of Medicine, Tucson, AZ, 85724, USA.7Center for Applied Genetics and Genomic Medicine, University of Arizona, Tucson, AZ, 85721, USA. [email protected] of Neurology, University of Arizona College of Medicine, Tucson, AZ, 85724, USA. [email protected].
Abstract
Among patients with intractable epilepsy, the most commonly performed surgical procedure is craniotomy for amygdalohippocampectomy (AH). Stereotactic laser amygdalohippocampotomy (SLAH) has also been recently employed as a minimally invasive treatment for intractable temporal lobe epilepsy (TLE). Among patients treated with AH and SLAH approximately 65% and 54% of patients become seizure-free, respectively. Therefore, selection criteria for surgical candidates with improved prognostic value for post-operative seizure-free outcome are greatly needed. In this study, we perform RNA sequencing (RNA-Seq) on whole blood leukocyte samples taken from 16 patients with intractable TLE prior to SLAH to test the hypothesis that pre-operative leukocyte RNA expression profiles are prognostic for post-operative seizure outcome. Multidimensional scaling analysis of the RNA expression data indicated separate clustering of patients with seizure free (SF) and non-seizure-free (NSF) outcomes. Differential expression (DE) analysis performed on SF versus NSF groups revealed 24 significantly differentially expressed genes (≥2.0-fold change, p-value < 0.05, FDR <0.05). Network and pathway analyses identified differential activation of pathways involved in lipid metabolism, morphology of oligodendrocytes, inflammatory response, and development of astrocytes. These results suggest that pre-operative leukocyte expression profiles have prognostic value for seizure outcome following SLAH.
www.ncbi.nlm.nih.gov/pubmed/30705324
|
